ФИШЕР Лоран Бернар (FR),КАРБУЛЕК Никола Пьер Ив (FR),ЛЮКС Фабьен (FR)
申请号：
RU2015111255
公开号：
RU2015111255A
申请日:
2013.08.30
申请国别(地区):
RU
年份:
2016
代理人:
摘要：
1. A composition comprising a chimeric protein consisting of Leishmania antigens, kinetoplastid membrane protein 11 (KMP11), sterol methyl transferase (SMT), A2 and cysteine proteinase (CP) .2. The composition of claim 1, wherein the chimeric protein is at least 90% identical to the sequence of SEQ ID NO: 2.3. The composition of claim 1, wherein the chimeric protein is encoded by a polynucleotide identical to at least 90% of the sequence of SEQ ID NO: 1.4. A composition according to claim 1, wherein the chimeric protein is substantially solubilized in an aqueous solution or substantially refolded. The composition of claim 1, wherein the chimeric protein is solubilized or refolded using high pressure. The composition of claim 5, wherein the high pressure is from about 1000 bar to about 5000 bar. The composition of claim 6, wherein high pressure is applied for at least 20 hours. A composition according to claim 5, wherein the chimeric protein is solubilized or refolded from E. coli inclusion bodies. The composition of claim 8, wherein the E. coli inclusion bodies are prepared in a buffer not containing or containing reduced urea. The composition of claim 8, wherein the E. coli inclusion bodies are prepared in a buffer containing dithiothreitol (DTT). A composition according to claim 9 or 10, wherein the inclusion bodies of E. coli are additionally subjected to high pressure in the range from about 1000 bar to about 5000 bar. The composition of claim 11, wherein the E. coli inclusion bodies are treated at high pressure for from about 20 hours to about 100 hours. The composition of claim 12, wherein the inclusion bodies of E. coli undergo pressure reduction at a rate of about 83 bar / h-125 bar / h. The composition of claim 5, wherein the high pressure rises in a stepwise manner. A method for treating or preventing leishmaniasis in an animal susceptible to a leishamania infection by inducing an immune response in an animal against1. Композиция, включающая химерный белок
