FISCHER LAURENT BERNARD,CARBOULEC NICOLAS PIERRE YVES,LUX FABIEN
申请号：
NZ70628813
公开号：
NZ706288A
申请日:
2013.08.30
申请国别(地区):
NZ
年份:
2016
代理人:
摘要：
Methods for producing a soluble protein expressed in prokaryotes or eukaryotes comprising the steps of (i) preparing inclusion bodies in a buffer comprising 0M to about 2M urea to form an inclusion body suspension； (ii) subjecting the inclusion body suspension to a target high pressure consisting of a stepwise increase of pressure over a period of time； and (iii) maintaining the high pressure applied to the inclusion bodies for a period of time.
