An antrodia camphorata culturing method for increasing the content of antrodia camphorata acid C and the content of zhankuic acid C comprises the steps that a antrodia basswood is provided an antrodia camphorata bacterium is provided and inoculated to the antrodia basswood, so that the antrodia basswood per kilo contains, by dry weight, 0.162 g of hyphae and mycelium pellets a light source with the wavelength being 600 nm-700 nm is used for irradiating the antrodia basswood where the antrodia camphorata bacterium is inoculated to obtain an antrodia camphorata sporophore, wherein the antrodia basswood where the antrodia camphorata bacterium is inoculated is irradiated by the light source for 8-12 hours per day, and continuous irradiation is performed for 30-40 days. According to the antrodia camphorata culturing method, a lot of antrodia camphorata acid C and zhankuic acid C can be produced, and therefore the nutritive value of antrodia camphorata is increased. In addition, the culturing time of the antrodia camphorata can be shortened, culturing cost is lowered, and the mass production of the antrodia camphorata is facilitated.
