A biomedical detection device includes a sample cell, an electromagnetic generating unit, a light source and a light detection unit. The sample cell can be filled with a liquid sample containing a detection object and a magnetic biosensor capable of combining the detection object to form a magnetic cluster. The electromagnetic generating unit is disposed at opposite two sides of the sample cell, and capable of generating an oscillating magnetic field of single frequency to drive motions of the magnetic biosensor and the magnetic cluster. The light source is disposed at a side of the sample cell, and capable of emitting light rays to pass through the liquid sample. The light detection unit is disposed at another side of the sample cell oppositely to the light source, and capable of outputting electrical signals based on a change of the light rays.
