The present invention relates to a composition for promoting stem cell proliferation which contains a vegetable peptone. More specifically, this invention relates to: a serum-free composition for culturing stem cells which contains a vegetable peptone a composition for improving skin condition containing the vegetable peptone as an active ingredient and a composition for improving skin condition containing a culture medium as an active ingredient in which cultured stem cells are removed after being cultured in a serum-free composition medium for stem cell culture containing the vegetable peptone. Since the disclosed serum-free composition for culturing stem cells does not need the use of expensive animal serum, the manufacturing cost can be remarkably lowered. Also the use of anima serum in the medium can basically prevent contamination material caused by the use of animal serum. In addition, the disclosed composition containing the vegetable peptone and the medium in which the stem cells are cultured in the serum-free medium both serve to promote and activated the proliferation of stem cells, and improve various skin conditions.
