COLLEGE OF MEDICINE POCHON CHA UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION
发明人:
LEE, SU MAN,이수만,PARK, SOO JEONG,박수정,LIM, DAE SEOG,임대석,LEE, YUN MI,이윤미,LEE, SUN HEE,이선희
申请号：
KR1020110014329
公开号：
KR1020120094981A
申请日:
2011.02.18
申请国别(地区):
KR
年份:
2012
代理人:
摘要：
PURPOSE: A pharmaceutical composition for preventing or treating osteoblast and a differentiation method of mesenchyme stem cell to osteonosus are provided to effectively promote differentiation of the mesenchyme stem cell to the osteonosus, thereby effectively manufacturing osteoblast without any safety problem and sinking calcium.CONSTITUTION: A pharmaceutical composition for preventing or treating osteoblast comprises mesenchyme stem cell, fucoidan, suramin, or a differentiating agent of a compound represented by chemical formula 2. The differentiating agent is the compound of the chemical formula 2 or suramin. 10^6~10^9 cells/ml concentration of the mesenchyme stem cell and 1-10uM concentration of the differentiating agent are existed. The differentiating agent is fucoidan. The mesenchyme stem cell is existed as the concentration of 10^6-10^9 cells/ml and the differentiating agent is existed as the concentration of 0.1-1 mg/ml. The mesenchyme stem cell shows CD73 positivity, CD90 positivity, CD105 positivity and CD166 positivity. The mesenchyme stem cell is a marrow, umbilical cord blood, placenta or fat derivative mesenchyme stem cell. A differentiation method of the mesenchyme stem cell to the osteoblast cultures the mesenchyme stem cell under the presence of the compound of Suramin or the chemical formula 2 as a differentiation promotor and differentiate the mesenchyme stem cell into the osteoblast.COPYRIGHT KIPO 2012본 발명은 중간엽 줄기 세포 및 푸코이단, 수라민, 및 화학식 2의 화합물(NF157)로 이루어진 군으로부터 1종 이상 선택된 분화유도제를 포함하는, 골질환의 예방 또는 치료용 약학 조성물을 제공한다. 또한, 본 발명은 분화유도제로서 수라민 또는 화학식 2의 화합물(NF157)을 사용하여 중간엽 줄기 세포를 골아세포(osteoblast)로 분화시키는 것을 특징으로 하는, 중간엽 줄기 세포의 골아세포로의 분화방법을 제공한다.
