KWON SE CHANG,KIM DAE JIN,JANG MYUNG HYUN,KIM SEUNG SU,LEE JONG SOO,CHOI JAE HYUK
申请号：
NZ62433812
公开号：
NZ624338A
申请日:
2012.11.02
申请国别(地区):
NZ
年份:
2016
代理人:
摘要：
Disclosed is a method for preparing a conjugate of a physiologically active polypeptide covalently bound to a non-peptide polymer, comprising: (1) reacting physiologically active polypeptide with two or more N-terminals with a non-peptide polymer with reactive groups on 2-3 ends in a reaction solution with a pH of 4.5-7.0 comprising 45-55% organic solvent and sodium citrate to link physiologically active polypeptide with non-peptide polymer； and (2) isolating and purifying the conjugate of physiologically active polypeptide covalently bound to non-peptide polymer from the reaction mixture of the step (1).
