GALLI, ROBERTA,PREUSSE, GRIT,KOCH, EDMUND,STEINER, GERALD,KRAUTWALD-JUNGHANNS, MARIA-ELISABETH,BARTELS, THOMAS
申请号：
CA2953813
公开号：
CA2953813A1
申请日:
2015.07.03
申请国别(地区):
CA
年份:
2016
代理人:
摘要：
The invention relates to a method for the Raman spectroscopic, in ovo sex determination of fertilised and incubated birds' eggs (1), wherein the embryo, including the extra-embryonic structures, can move in the egg, and is not yet attached to the shell at the time of measuring. In addition, the following steps are carried out: monitoring the time course of the incubated egg until at least one recognisable blood vessel (21) is formed； creating a hole (2) in the shell in the region near to the attached blood vessel, using a hole-generating unit； finding the blood vessel forming in the egg using a vision system (19, 13) and a coaxial or lateral illumination with light (10a) in the visible wavelength range； positioning at least one blood vessel in the laser focus of a laser source (3), either by moving the egg or moving a lens (6) of a device (5) for introducing the laser light (3a), and detecting the Raman scattered radiation (7)； registering the Raman scattered radiation of the irradiated blood vessel using the device for introducing the laser light, and for detecting the Raman scattered radiation, wherein, during the measuring process, a movement of the blood vessel out of the focus can be avoided by tracking using the vision system； evaluating the Raman scattered radiation in an evaluation unit； determining and displaying the sex of the embryo in the bird's egg.
