The present invention relates to a method for freezing and thawing adipose stem cells, and more specifically, to a method for obtaining stem cells with improved survival rates and high activity by freezing adipose stem cells using DMSO and anti-freeze protein and gradually thawing frozen adipose stem cells. A method for storing adipose stem cells according to one embodiment of the present invention includes a step of making adipose stem cells into a DMSO and anti-freeze protein suspension, and a step of freezing the suspension.COPYRIGHT KIPO 2014본 발명은 지방유래 줄기세포를 DMSO 및 항동결 단백질을 이용하여 냉동시키고, 냉동된 지방유래 줄기세포를 단계적으로 해동시켜서 생존율 및 활성이 높은 줄기세포를 수득하는 방법에 관한 것이다.