The method that regeneration of cartilage is assessed in embodiment includes that will place in the presence of the cell mass what culture medium for containing auricular cartilage cell, the liquid component of at least part taken from prior culture media by Hou therewith, and measure GFAP content contained in the aforementioned liquid component taken, and based on aforementioned GFAP content, judge the regeneration of cartilage that obtains or can obtain from foregoing population if appropriate for transplanting.實施方式中評估再生軟骨之方法包含將含有耳廓軟骨細胞之細胞群於培養基存在下進行放置，與之後自前述培養基中採取至少一部分之液體成分，及測定已採取之前述液體成分中所含之GFAP含量，以及以前述GFAP含量為基礎，判斷自前述細胞群獲得或可得之再生軟骨是否適合移植。
