An injection apparatus (1) is described for making an injection at a predetermined depth in skin comprising:a skin positioning member (24) for positioning on a patch of skin (90) within an area of skin (92) such that at least a part of said patch of skin may be held elevated above or depressed below said area of skin,an injection needle (50), andmeans guiding the injection needle to slide beneath the skin positioning member to an injection position in which the distal end (52) of the needle lies at a predetermined distance below the skin positioning member.An injection apparatus is also described comprising a detachable marker unit (4) and means for securing said marker unit at an injection site prior to the making of an injection to mark the position of the injection site.