The present invention discloses a plant growth regulator. The effective ingredients of the plant growth regulator comprise active components A and B, wherein the active component A is thidiazuron, and the active component B is amino-oligosaccharin. Also provided are a preparation method and use of the composition. Experimental results indicate that the composition of the present invention is effective in regulating the growth, increasing the disease and stress resistance, promoting the health and increasing the yield and quality of crops.