A culture method of a fruiting body of Antrodia camphorata is provided, which includes: (a) fermenting a culture medium containing yeast at 5-35° C. for 3-30 days (b) adding wood flour to the fermented culture medium with stirring (c) placing the wood flour and the culture medium into a vessel (d) sterilizing the vessel containing the wood flour and the culture medium (e) inoculating Antrodia camphorata strains into the sterilized vessel containing the wood flour and the culture medium, and culturing at 5-35° C., to form mycelia (f) inoculating the wood flour containing Antrodia camphorata mycelia into a wood segment (g) placing the wood segment inoculated with Antrodia camphorata mycelia in an environment where the temperature is 5-35° C. and the humidity is 65-85%, and culturing for a period of time and (h) removing the wood flour remained on the wood segment, then placing the wood segment in an environment where the temperature is 15-35° C. and the humidity is 80-98%, and culturing for a period of time, to form a fruiting body of Antrodia camphorata. A culture method of Antrodia camphorata mycelia, a culture method of a fruiting body of Antrodia camphorata, and a fruiting body of Antrodia camphorata cultured by the same method are also provided. The fruiting body of Antrodia camphorata cultured by the method of the present invention is thick and solid, and the content of triterpenoids is comparable to that of wild Antrodia camphorata.本發明係關於一種樟芝子實體培養方法,其係包含下列步驟:(a)取一含酵母之培養基,於5-35℃下發酵3-30天;(b)加入木屑於前述發酵後之培養基並攪拌;(c)將前述木屑及培養基置於一器皿中;(d)將前述裝有木屑及培養基之器皿滅菌;及(e)取一樟芝菌種接種至前述滅菌過之含有木屑及培養基之器皿中,並於5~35℃下培養形成菌絲體;(f)將前述含有樟芝菌絲體之木屑接種至一段木;(g)將前述已接種菌種之段木置於一溫度5~35℃、濕度65~85%之環境下培養一段時間;及(h)清除段木上殘餘之木屑後,將段木置溫度15~35℃、濕度80~98%的環境下栽培一段時間後即可形成樟芝子實體。本發明亦提供一種樟芝菌絲體之培養方法、一種樟芝子實體之培養方法及一種由前述樟芝子實體培養方法所培養而成之樟芝子實體。利用本發明之方法所培養之樟芝子實體,具有厚實子實體,且其三萜類之含量與野生樟芝相近。
