Федеральное государственное унитарное предприятие "Государственный научно-исследовательский институт особо чистых биопрепаратов" Федерального медико-биологического агентства (RU)
发明人:
Вербицкая Наталья Борисовна (RU),Добролеж Ольга Васильевна (RU),Кобатов Алексей Иванович (RU),Петров Леонид Николаевич (RU),Вахитов Тимур Яшэрович (RU),Полевая Елена Валерьевна (RU)
申请号：
RU2012140941/10
公开号：
RU2012140941A
申请日:
2012.09.26
申请国别(地区):
RU
年份:
2014
代理人:
摘要：
1. A process for preparing a probiotic product comprising culturing Lactobacillus acidophilus strains in liquid medium, the introduction of protecting the drying medium containing gelatin, ascorbic acid, sucrose, skimmed milk and water, freezing, freeze-drying and the preparation of the formulation, wherein that the cultivation is carried out on skim milk with 0.4% yeast extract using strains of Lactobacillus acidophilus L-75 and D-76, and after culturing in the culture fluid is introduced to the complex of carboxylic slot containing lactic acid - 10-15% by weight, succinic acid, 5-10% by weight of sodium formate -.. 5-10% by weight of sodium acetate -. 67% mass.2. A method according to claim 6, characterized in that the initial ratio of strains D-75 and D-76 at seeding varies in the range 1: 2-2: 1.3. A method according to claim 1, characterized in that the liquid introduced into the culture complex carboxylic acids additionally contains 10-15% by weight. aminokislot.4. A method according to claim 1, characterized in that the liquid introduced into the culture complex carboxylic acids additionally contains 10-15% by weight. lactic kisloty.5. A method according to claim 1, characterized in that the liquid introduced into the culture complex carboxylic acids additionally contains 0.01% by weight. B.6 group vitamins. A method according to claim 5, characterized in that the vitamins B complex carboxylic acids are administered 0.01% by mass. riboflavina.7. A method according to claim 5, characterized in that the vitamins B complex carboxylic acids are administered 0.02% by weight. Folic kisloty.8. Probiotic preparation, characterized in that it is obtainable by a method according to claim 1 and comprising a mixture of strains of Lactobacillus acidophilus, gelatin, sugar1. Способ получения пробиотического препарата, включающий в себя культивирование штаммов Lactobacillus acidophilus в жидкой питательной среде, введение защитной среды высушивания, содержащей желатин, аско
