abstract the present invention relates to a method of producing biosurfactants, such as surfactin, comprising culturing at least one biosurfactant-producing microbe in a liquid culture medium including vinasse as a carbon source. methods of using the crude biosurfactant containing culture broth in tertiary oil recovery and the antibacterial compositions in tertiary oil recovery are also described. methods of using the culture broth residue after isolation of the biosurfactants as fertilizer and compositions for this use are also described. abstract of the patent abstract: "method of producing biotensive agents" The present invention relates to a method of producing biotensive agents, such as surfactin, comprising culturing at least one biotensive producer producing microbe in a liquid culture medium comprising vinasse as a carbon source. Methods of using broth containing crude biotensive active in tertiary oil recovery and as antibacterial composition in tertiary oil recovery are also described. Methods of using the culture broth residue after isolation of the biotensive agents as fertilizer and compositions for this use are also described.abstract the present invention relates to a method of producing biosurfactants, such as surfactin, comprising culturing at least one biosurfactant-producing microbe in a liquid culture medium comprising vinasse as a carbon source. methods of using the crude biosurfactant containing culture broth in tertiary oil recovery and as antibacterial compositions in tertiary oil recovery are also described. methods of using the culture broth residue after isolation of the biosurfactants as fertilizer and compositions for this use are also described. tradução do resumo resumo patente de invenção: "método de produção de biotensoativos". a presente invenção refere-se a um método de produção de biotensoativos, tal como surfactina, compreendendo cultura de pelo menos um micróbio produtor de biotensoativo em um meio de cultura líquido compreenden