PROBLEM TO BE SOLVED: To provide minimalistic gene expression constructs, transfer thereof into cells and use thereof for gene expression for molecular-medicinal applications.SOLUTION: The invention provides a DNA construct for gene expression, the construct being a linear, open chained double stranded DNA comprising a promoter sequence, a coding sequence, a termination signal, and at least one L-DNA nucleotide. Further provided is the use of the above DNA construct for stable or transient transfection of a human, animal or eukaryotic cell, as well as for gene therapy or DNA vaccination.SELECTED DRAWING: Figure 1【課題】最小限の遺伝子発現構築物、細胞へのこの導入及び分子医学的応用のための遺伝子発現のためのこの使用。【解決手段】プロモーター配列、コード配列及び終結シグナルを含む線状の開鎖されたＤＮＡ二本鎖であり、少なくとも１つのＬ－ＤＮＡヌクレオチドを含む、遺伝子発現のためのＤＮＡ構築物。更には、ヒト、動物又は真核生物細胞の安定な又は一過性のトランスフェクションのための、及び遺伝子治療又はＤＮＡワクチン接種のための上記ＤＮＡ構築物の使用。【選択図】図１
