This Hair Ming Department Ti Gong Yi Seed there is inhibition fungi the rhizoma anemarrhenae extract of plant disease and Should, The rhizoma anemarrhenae Cui Qu Wu Department to be caused to be extracted by molten Elixirs and obtain, and The extracts include at least compound (I). The ability that there is good inhibition fungi to cause pseudomonas syringae pv. maculicola and anthrax bacteria by The rhizoma anemarrhenaes extract and Theization He Wu Department, and Zhi Wu Yuan Farming Drug System it can make in Will its Should Yong Yu Farming industry, to inhibit disease Shi Seed plant persons to exempt from what Shou Cheng Damage Shi I, also not the pollution of the borders Hui Zao Cheng Ring, disruption of ecological balance, soil deterioration etc. bad shadow Ring.本發明係提供一種具有抑制真菌引起植物疾病之知母萃取物及應用,該知母萃取物係由溶劑萃取而得,且該萃取物至少包含化合物(I)。該知母萃取物及該化合物係具有良好的抑制真菌引起十字花科黑斑病菌及炭疽病菌之能力,且可將其應用於農業上植物源農藥之製作,以抑制病害使種植者免於收成損失之餘,亦不會造成環境污染、生態失衡、土壤劣化等不良影響。