Provided is an information processing apparatus which supports diagnosis based on a tissue specimen image obtained by capturing body tissues, including an area generation unit that divides at least one feature value of the tissue specimen image into a plurality of levels based on a magnitude of the feature value, and generates an area on the tissue specimen image belonging to each level; and an overlay image generation unit that associates the area of each level which is generated by the area generation unit with an image which is processed to have the same shape and the same positional relation as the area and in which a magnitude relation of the feature value is identifiable and generates an overlay image.