F gene-deficient virus virions are successfully recovered byusing an F gene-deficient Sendai virus genomic cDNA. Further, Fgene-deficient infectious viral particles are successfullyconstructed by using F-expressing cells as helper cells . Also, F geneand HN gene-deficient virus virions are successfully recovered byusing a virus genomic cDNA deficient in both F gene and HN gene.Further, F gene and HN gene-deficient infectious viral particles aresuccessfully produced by using F- and HN-expressing cells as helpercells . A virus deficient in F gene and HN gene and having F proteinis constructed by using F-expressing cells as helper cells. Further,a VSV-G pseudo type virus is successfully constructed by usingVSV-G-expressing cells . Techniques for constructing these deficientviruses contribute to the development of vectors of Paramyxoviridaeusable in gene therapy.
