A pesticidal protein class exhibiting toxic activity against Coleopteran and Lepidopteran pest species is disclosed, and includes, but is not limited to, TIC7040, TIC7042, TIC7381, TIC7382, TIC7383, TIC7386, TIC7388, and TIC7389. DNA constructs are provided which contain a recombinant nucleic acid sequence encoding the TIC7040, TIC7042, TIC7381, TIC7382, TIC7383, TIC7386, TIC7388, and TIC7389 pesticidal proteins. Transgenic plants, plant cells, seed, and plant parts resistant to Coleopteran and Lepidopteran infestation are provided which contain recombinant nucleic acid sequences encoding the TIC7040, TIC7042, TIC7381, TIC7382, TIC7383, TIC7386, TIC7388, and TIC7389 pesticidal proteins of the present invention. Methods for detecting the presence of the recombinant nucleic acid sequences or the proteins of the present invention in a biological sample, and methods of controlling Coleopteran and Lepidopteran species pests using the TIC7040, TIC7042, TIC7381, TIC7382, TIC7383, TIC7386, TIC7388, and TIC7389 pesticidal proteins are also provided.Se describe una clase de proteínas pesticidas que presentan actividad tóxica contra especies de plagas de coleópteros y lepidópteros y que incluye, de modo no taxativo, TIC7040, TIC7042, TIC7381, TIC7382, TIC7383, TIC7386, TIC7388 y TIC7389. Se proporcionan construcciones de ADN que contienen una secuencia de ácido nucleico recombinante que codifica las proteínas pesticidas TIC7040, TIC7042, TIC7381, TIC7382, TIC7383, TIC7386, TIC7388 y TIC7389. Se proporcionan plantas transgénicas, células vegetales, semillas y partes de plantas resistentes a infestación por coleópteros y lepidópteros, que contienen secuencias de ácido nucleico recombinante que codifican las proteínas pesticidas TIC7040, TIC7042, TIC7381, TIC7382, TIC7383, TIC7386, TIC7388 y TIC7389 de la presente invención. También se proporcionan métodos para detectar la presencia de las secuencias de ácido nucleico recombinante o las proteínas de la presente invención en una m
