A class of pesticidal proteins is described that have toxic activity against species of beetles of beetles and lepidoptera and that includes, non-taxatively, TIC7040, TIC7042, TIC7381, TIC7382, TIC7383, TIC7386, TIC7388 and TIC7389. DNA constructs are provided that contain a recombinant nucleic acid sequence encoding the pesticidal proteins TIC7040, TIC7042, TIC7381, TIC7382, TIC7383, TIC7386, TIC7388 and TIC7389. Transgenic plants, plant cells, seeds and parts of plants resistant to beetle and lepidoptera infestation are provided,containing recombinant nucleic acid sequences encoding the pesticidal proteins TIC7040, TIC7042, TIC7381, TIC7382, TIC7383, TIC7386, TIC7388 and TIC7389 herein. Methods are also provided to detect the presence of recombinant nucleic acid sequences or proteins present in a biological sample, as well as methods to control pests of beetle and lepidopteran species with the pesticidal proteins TIC7040, TIC7042, TIC7381, TIC7382 , TIC7383, TIC7386, TIC7388 and TIC7389. Composition. Product.Se describe una clase de proteínas pesticidas que presentan actividad tóxica contra especies de plagas de coleópteros y lepidópteros y que incluye, de modo no taxativo, TIC7040, TIC7042, TIC7381, TIC7382, TIC7383, TIC7386, TIC7388 y TIC7389. Se proporcionan construcciones de ADN que contienen una secuencia de ácido nucleico recombinante que codifica las proteínas pesticidas TIC7040, TIC7042, TIC7381, TIC7382, TIC7383, TIC7386, TIC7388 y TIC7389. Se proporcionan plantas transgénicas, células vegetales, semillas y partes de plantas resistentes a infestación por coleópteros y lepidópteros, que contienen secuencias de ácido nucleico recombinante que codifican las proteínas pesticidas TIC7040, TIC7042, TIC7381, TIC7382, TIC7383, TIC7386, TIC7388 y TIC7389 de la presente. También se proporcionan métodos para detectar la presencia de las secuencias de ácido nucleico recombinante o las proteínas de la presente en una muestra biológica, así como métodos para controlar
