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Phamaceutial composition comprising of dichloromethane fraction of Aster yomena for immune enhancing
专利权人:
REPUBLIC OF KOREA(MANAGEMENT ; RURAL DEVELOPMENT ADMINISTRATION)
发明人:
황경아,송진,황유진,최정숙,노건민,황인국,HWANG, KYUNG AKR,SONG, JINKR,HWANG, YU JINKR,CHOE, JEONG SOOKKR,NOH, GEON MINKR,HWANG, IN GUKKR
申请号:
KR1020160044993
公开号:
KR1017463880000B1
申请日:
2016.04.12
申请国别(地区):
KR
年份:
2017
代理人:
摘要:
The present invention relates to a pharmaceutical composition for enhancing immunity comprising a dichloromethane fraction of Asteryomena (AY) extract as an active ingredient. More specifically, the present invention relates to a pharmaceutical composition for enhancing immunity comprising dichloromethane (CH 2 Cl 2 ), ethanol (EtOH) (Hexane), ethyl acetate (EtOAc) and butanol (BuOH) fractions were measured. The dichloromethane fraction showed no cytotoxicity and the macrophage proliferation effect was superior to the other fractions. Immunoreactivity The production of cytokines, interleukin-6 and TNF-α was about 50% of that of the positive control (LPS). Further, the increase in the expression level of the Fc receptor was measured. As a result, Methane fraction showed an increase in the expression level of Fc receptor I about 2.5 times and the highest expression level in the solvent fraction than in the control group. As a result, it was confirmed that the dichloro Carbon fraction can be effectively used as a pharmaceutical composition for immune enhancement.본 발명은 쑥부쟁이(Aster yomena, AY) 추출물의 디클로로메탄 분획물을 유효성분으로 포함하는 면역증강용 약학적 조성물에 관한 것으로, 구체적으로 쑥부쟁이의 디클로로메탄(CH2Cl2), 에탄올(EtOH), 헥산(Hexane), 에틸아세테이트(EtOAc) 및 부탄올(BuOH) 분획물의 면역 활성을 측정한 결과, 디클로로메탄 분획물이 세포 독성이 없으며, 다른 분획물과 비교하여 대식세포 증식 효과가 가장 우수하였고, 면역관련 사이토카인인 IL(interleukin)-6 및 TNF-α의 생성량이 양성대조군(LPS; lipopolysaccharide)의 50% 정도의 값을 나타냈으며, 또한, Fc 수용체의 발현량 증가를 측정한 결과, 쑥부쟁이의 디클로로메탄 분획물이 대조군보다 Fc 수용체 I의 발현량이 약 2.5배 증가하였고, 용매 분획물 중 가장 높은 발현량을 나타냄을 확인함으로써, 본 발명의 쑥부쟁이 추출물의 디클로로메탄 분획물은 면역증진용 약학적 조성물로 유용하게 사용될 수 있다.
来源网站:
中国工程科技知识中心
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