The present disclosure provides recombinant nucleic acids comprising one or more polynucleotides encoding a cystic fibrosis transmembrane conductance regulator (CFTR) polypeptide (e.g., a human CFTR polypeptide)； viruses comprising the recombinant nucleic acids； compositions and formulations comprising the recombinant nucleic acids and/or viruses； methods of their use (e.g., for the treatment of a chronic lung disease, such as cystic fibrosis)； and articles of manufacture or kits thereof.
