A cell isolation apparatus includes: a cylinder member that is inserted into a container into which a reagent is to be introduced a net which is stretched over the cylinder member a tissue acquiring unit that is projected from the cylinder member and a bottom lid portion that is configured by a bottomed pipe in which a first hole that communicates an interior with an exterior is formed, and that is fitted to a bottom portion of the cylinder member in a state where the net and the tissue acquiring unit are accommodated.
