A heating and cooling mat assembly includes a mat having a bottom wall, a top wall and a perimeter wall. A conduit is positioned in the man and has fluid therein. A pump is in fluid communication with the conduit to pump the fluid through the conduit. A heat emitter is thermal communication with the liquid in the conduit. The heat emitter warms the liquid when the heat emitter is turned on. A cartridge is removably couplable to the mat. The cartridge includes a pair of first ports. The conduit is in fluid communication with a pair of second ports. Each of the first ports is removably coupled to one of the second ports to fluidly couple the conduit to the cartridge. The cartridge is fillable with a cold fluid so that the conduit is cooled when the pump is turned on and the cartridge is coupled to the conduit.