An image pickup apparatus includes first and second irradiation units that emit first light and second light, respectively, an image pickup unit that outputs, as pixel information, an electrical signal after photoelectric conversion from a pixel arbitrarily designated as a read target among pixels, a setting unit that arbitrarily sets a pixel as the read target and a read order in the image pickup unit, a control unit that controls irradiation processes in the first and second irradiation units and changes the pixel as the read target and the read order according to a type of an irradiation unit that emits light, a reading unit that reads the pixel information by causing the pixel information to be output from the pixel set as the read target, in accordance with the read order, and an image processing unit that generates an image from the pixel information read by the reading unit.