An image pickup apparatus includes: a plurality of image pickup devices configured to pick up images of a same object and sequentially read the images for each line; an objective optical system configured to form the images at different image-forming positions on the image pickup devices; a memory configured to record predetermined time differences for reading the leading positions of the effective pixels of the images at the same timing; and an I2C control circuit configured to control the image reading timing of the image pickup devices by generating a plurality of synchronizing signals based on a master synchronizing signal and supplying the synchronizing signals to the image pickup devices after shifting the synchronizing signals by the respective predetermined time differences.