The present invention provides a bio-cellulose membrane and the production method thereof, the bio-cellulose membrane having a first surface, a second surface and a loose layer, wherein the average pore size of the first surface and second surface are larger than the loose layer. The bio-cellulose membrane exhibits excellent water retention effect.本發明提供一種具優異保水效果之生物纖維膜及其製造方法，該生物纖維膜具有一第一表面、一第二表面及一疏鬆層，其中該第一表面及該第二表面之平均孔徑小於該疏鬆層。
