FIELD: pharmaceutical industry.SUBSTANCE: invention relates to pharmaceutical industry, namely to process for obtaining of a hyaluronidase preparation. Method for obtaining the hyaluronidase preparation includes grinding the testes of cattle, extraction with acetic acid with constant stirring, centrifuging the extract, concentrating the resulting centrifugate, diafiltration, Sterilizing filtration, ampouling and freeze-drying under certain conditions. Preparation of hyaluronidase.EFFECT: above described method allows to increase the yield and activity of the final product.4 cl, 5 dwg, 7 ex, 1 tbl