UNIVERSIDAD NACIONAL AUTONOMA DE MEXICO;ARIZONA BOARD OF REGENTS ON BEHALF OF THE UNIVERSITY OF ARIZONA
发明人:
Tabashnik, Elliot Bruce,Bravo-De La Parra, Maria Alejandra,Soberon-Chavez, Mario,Pardo-Lopez, Liliana
申请号：
AU2007354729
公开号：
AU2007354729B2
申请日:
2007.06.08
申请国别(地区):
AU
年份:
2012
代理人:
摘要：
The invention relates to a method for obtaining DNA constructions coding forCry toxins with 3 domains (also known as Cry toxins, Bt toxins or δ-endotoxins),which lack helix α-1. Said DNA constructions were modified in order toencode proteins that can kill insects that are resistant to the correspondingnon-modified Cry toxins. The invention relates to the DNA constructions of thegenes of modified 3-domain Cry toxins and the modified 3-domain Cry proteins,together with methods, molecular vectors and host cells containing said constructions,as well as recombinant methods for producing the modified 3-domain Cry toxins.In addition, the invention relates to formulations containing the modified3-domain Cry toxins. Resistance of insects to non-modified Cry toxins is dueto a reduced binding of the toxin to the receptors in the insect's intestine.More specifically, the invention relates to the expression of the modified 3-domainCry toxins, the methods for expressing said modified 3-domain Cry toxins, transformedmicroorganisms and transgenic plants with constructions that express the modified3-domain Cry toxins and methods for suppressing resistance of pests to non-modifiedCry toxins.
