The present invention is directed to a bioconjugate vaccine such as an O1-bioconjugate vaccine, comprising: a protein carrier comprising an inserted consensus sequence, D/E - X - N - Z - S/T, wherein X and Z may be any natural amino acid except proline； and at least one antigenic polysaccharide from at least one bacterium, linked to the protein carrier, wherein the at least one antigenic polysaccharide is a bacterial O-antigen from one or more strain of Shigella, E. coli or Pseudomonas aeruginosa； and, optionally, an adjuvant. In another aspect of the present invention is directed to a method of producing a O1- bioconjugate in a bioreactor comprising a number of steps.
