A method and system for UV detection of sterilant concentration and dissipation in a volume of a chamber may comprise focusing cameras on at least one point of an object in the chamber； transmitting UV light and sterilant into the chamber； scanning, using the cameras, the at least one point of the object and determining an amount of absorbance at the points； calculating, using the amount of absorbance, a concentration of the sterilant for each of the one or more points； and when the concentration is greater than a threshold, removing the sterilant from the volume. The sterilant may be hydrogen peroxide. The cameras may be stereoscopic cameras. The chamber may be partitioned into a grid of voxels for scanning.
