Cartridge for volatile substances in the liquid state, such as insecticides or fragrances, of the type including a tray-shaped container closed on one side with a multilayer film, the multilayer film consisting of at least one internal vapour permeable membrane and one external vapour barrier layer. A directing/assisting unit is provided, apt to direct/assist the removal of just a first portion of the external vapour barrier layer—in the form of notches, weakening lines or strengthening lines—along a desired partition line, for cartridge activation.