Lancing apparatus 1 includes a puncture needle 31 that punctures the living tissue, a shaft portion 33, the piercing member (3) having a coupling portion 32 for coupling the puncture needle 31 and the shaft portion 33, the longitudinal shape to be inserted into the urethra and urethra insertion member 4, it is equipped with a vaginal insertion member 5 of longitudinal shape to be inserted into the vagina, these puncture member 3, and a support member for supporting the urethra insertion member 4 and vaginal insertion member 5. Piercing needle 31 has a sharp needle point at the tip, it is curved in an arc around the shaft portion 33. Shank 33 is mounted rotatably on the support member. The support member, when the piercing member 3 was punctured biological tissue rotates, the needle tip of the puncture needle 31 distally from the urethra insertion member 4 or the center 311 of the puncture needle 31 than its extension, namely the urethra so as to pass below the insertion member 4 or its extension, thereby restricting the positional relationship between the puncture member 3 and the urethra insert 4.穿刺装置1は、生体組織を穿刺する穿刺針31と、軸部33と、穿刺針31と軸部33を連結する連結部32とを有する穿刺部材3と、尿道内に挿入される長手形状の尿道挿入部材4と、膣内に挿入される長手形状の膣挿入部材5と、これら穿刺部材3、尿道挿入部材4および膣挿入部材5を支持する支持部材とを備えている。穿刺針31は、先端に鋭利な針先を有し、軸部33を中心とする円弧状に湾曲している。軸部33は、支持部材に回動自在に設置されている。支持部材は、穿刺部材3が回動して生体組織を穿刺したとき、穿刺針31の針先が、尿道挿入部材4またはその延長線よりも穿刺針31の中心311から遠位側、すなわち尿道挿入部材4またはその延長線の下方を通過するように、穿刺部材3と尿道挿入部材4との位置関係を規制している。