The present invention relates to methods of wound debridement. Particularly, the present invention relates to methods of debridement of chronic wounds comprising topically applying to a wound site a debriding formulation in the form of a hydrogel comprising a proteolytic enzyme mixture obtained from bromelain and a water-soluble gelling agent, the debriding formulation being applied to the wound site up to ten times over a period of up to four weeks, thereby achieving debridement of chronic wounds.本發明係關於傷口清創之方法。特別地,本發明係關於慢性傷口之清創方法,其包含局部施加至傷口位點包含自鳳梨酶獲得之蛋白分解酶混合物及水溶性膠凝劑之呈水凝膠形式之清創調配物,經多至四週之時段將該清創調配物施加至該傷口位點多至十次,藉此達成慢性傷口之清創。