PROBLEM TO BE SOLVED: To provide a manufacturing method of a toothbrush in which a step of attaching a toothbrushing part to a handle part is not necessary.SOLUTION: A manufacturing method of a toothbrush 1 including a handle part 10 and a toothbrushing part 20 consisting of a plurality of linear strips 22 comprises a preparation step and a slitting step. The preparation step is a step of preparing a paper shaft 30 in which a tip part 32 becomes the toothbrushing part 20 and the remnant part 34 becomes the handle part 10. The slitting step is a step of cutting the tip part 32 of the paper shaft 30 prepared by the preparation step in the radial direction thereof to separate the tip part 32 into the linear strips 22.SELECTED DRAWING: Figure 8