An object guide system includes a plurality of light projecting devices, at least one image capture device, a control device electrically coupled with the light projecting devices, and a display unit electrically coupled with the at least one image capture device and the control device. When an object enters an operation space, a projected information is imaged on the object. Then, the at least one image capture device captures the cross information of the operation space and the three-dimensional position information of the object. Then, the control device calculates the coordinates of the object that is moving in the operation space. Thus, after the at least one image capture device captures the cross information of the operation space and the three-dimensional position information of the object, the object guide system proceeds interactive and guiding operations.