THE PRESENT INVENTION PROVIDES A METHOD OF MICROPROPAGATING A MONOCOTYLEDONOUS PLANT COMPRISING: (A) CULTIVATING AN EXPLANT OF TISSUE FROM A MONOCOTYLEDONOUS PLANT SHOOT TIP ON A PRIMARY MEDIUM, WHEREIN THE EXPLANT HAS BEEN PRETREATED WITH A COLD TEMPERATURE AND THE PRIMARY MEDIUM COMPRISES AUXIN OR AUXIN AND CYTOKININ, TO PRODUCE A TOTIPOTENT EMBRYOGENIC CELL CULTURE (B) TREATING THE TOTIPOTENT EMBRYONIC CELL CULTURE WITH A COLD TEMPERATURE (C) MAINTAINING THE TOTIPOTENT EMBRYOGENIC CELL CULTURE BY CULTIVATION ON A SECONDARY MEDIUM, WHEREBY A TOTIPOTENT EMBRYOGENIC CELL CULTURE OF A MONOCOTYLEDONOUS PLANT IS PRODUCED AND MAINTAINED AND (D) TRANSFERRING THE EMBRYOGENIC CELL CULTURE OF STEP (C) TO A TERTIARY, MEDIUM TO CONTINUE MULTIPLICATION AND TO PRODUCE A PLANTLET WITH ROOTS AND SHOOTS, THEREBY MICROPROPAGATING A MONOCOTYLEDONOUS PLANT. THE MICROPROPAGATION TECHNIQUES DESCRIBED HEREIN PROVIDE PLANTS FOR SUCH PURPOSES AS DEVELOPMENT OF ELITE PLANT LINES, PHYTOREMEDIATION AND BIOMASS PRODUCTION.
