This present invention disclosed a method for culturing Ophiocordyceps sinensis by inoculating a fungus inoculum onto a medium, culturing with a culturing condition in the dark room, and then culturing under the light for at least four weeks. According to the method for culturing O. sinensis disclosed in this present invention, the cultured O. sinensis has better growth rate and its composition and content of active ingredients are similar with wild O. sinensis.
