Wilmar (Shanghai) Biotechnology Research & Development Center Co., LTD
发明人:
Xu Jun,Xuan Yaoji,Li Jinmin
申请号：
US201415035105
公开号：
US10144919(B2)
申请日:
2014.11.04
申请国别(地区):
美国
年份:
2018
代理人:
St. Onge Steward Johnston & Reens, LLC
摘要：
The present application provides mutants of wild type phospholipase C (PLC) specific to phosphatidylcholine from Bacillus cereus. The related mutations include mutation of asparagine at position 63 to another amino acid, also including mutation of arginine at position 20 to histidine and of alanine at position 83 to aspartic acid. The present application also provides a nucleic acid molecule encoding the mutant, a vector containing the nucleic acid molecule, and a cell containing the nucleic acid molecule or vector. The present application also provides uses of the mutant, nucleic acid molecule vector, and cell.
