Stephen Gorfien;Glenn Godwin;Richard Fike;Paul Price;Joyce Dzimian;David A. Epstein;Dale Gruber
发明人:
Stephen Gorfien,Richard Fike,Glenn Godwin,Joyce Dzimian,David A. Epstein,Dale Gruber,Paul Price
申请号：
US12463351
公开号：
US08455246B2
申请日:
2009.05.08
申请国别(地区):
US
年份:
2013
代理人:
摘要：
The present invention provides a cell culture medium formulation that supports the in vitro cultivation, particularly in suspension, of mammalian cells, particularly epithelial cells and fibroblast cells, and methods for cultivating mammalian cells in suspension in vitro using these media. The media comprise a basal medium and a polyanionic or polyanionic compound, preferably a polysulfonated or polysulfated compound, and more preferably dextran sulfate. The present invention also provides chemically defined, protein-free eukaryotic cell culture media comprising an iron chelate and zinc, which is capable of supporting the growth (and particularly the high-density growth of mammalian cells) in suspension culture, increasing the level of expression of recombinant protein in cultured cells, and/or increasing virus production in cultured cells.
