The present invention relates to the pharmaceutical field, and in particular to a ginsenoside composition, and preparation method and use thereof. The ginsenoside composition comprises rare ginsenosides Rh4, Rk3, Rk1 and Rg5. By conducting a systematic research on the change in active ingredients during the steaming process of ginseng and applying response surface to design experiments so as to optimize the ginseng processing process, a processing method wherein there is the most significant change in ginsenoside ingredients relative to the original medicinal material, and especially a processing method for efficiently obtaining rare ginsenoside ingredients, is unexpectedly discovered in the present invention. Through later screening of pharmacological activities, the inventors further unexpectedly discovered that the processed ginseng product rich in rare ginsenosides has a significant efficacy in invigorating kidney yang.
