Provided are a new tubulin inhibitor and applications thereof, said tubulin inhibitor being a series of compounds based on substituted heterocyclic skeletons, targeting the colchicine binding sites of tubulin. The structure is as below: Formula (I), wherein: formula (A), n independently expresses an integer between 0 and 5, the condition being that n≤5, A represents mono- or poly-substituted groups, said groups being selected from the group consisting of H, C-Camide group, C-Cacyloxy group, C-Calkanoyl group, C-Calkoxycarbonyl group, C-Calkoxy group, C-Calkylamino group, C-Calkylcarboxyamino group, aroyl group, aralkanoyl group, carboxyl group, cyano group, halogen group, hydroxyl group, nitro group and methylthienyl group.R=
