PRETREATMENT METHOD OF ENVIRONMENTAL WATER SAMPLE, PRETREATMENT AGENT FOR ENVIRONMENTAL WATER SAMPLE AND ESTIMATION METHOD OF AMOUNT OF BIOTA OR ORGANISMS
PROBLEM TO BE SOLVED: To provide a technique for suppressing degradation of the nucleic acids included in an environmental water sample which is subjected to an environmental DNA analysis.SOLUTION: The present invention provides a pretreatment method of an environmental water sample for estimating the amount of biota or a particular organism in the place where the environmental water sample is collected, by analyzing the nucleic acids included in the environmental water sample collected from a given environment, the method being characterized by contacting the environmental water sample before subjected to a nucleic acid analysis to an antiseptic which contains a quaternary ammonium salt as an active ingredient. As quaternary ammonium salts, benzalkonium chloride, benzethonium chloride and didecyl chloride dimethylammonium are illustrated.SELECTED DRAWING: Figure 2【課題】環境DNA分析に供する環境水試料に含まれる核酸の分解を抑えるための技術を提供する。【解決手段】所定の環境から採取された環境水試料に含まれる核酸を分析することにより、前記環境水試料の採取場所における生物相又は特定の生物の量を推定するための環境水試料の前処理方法であって、核酸の分析に供する前の前記環境水試料を、4級アンモニウム塩を有効成分とする消毒剤に接触させることを特徴とする環境水試料の前処理方法が提供される。4級アンモニウム塩として塩化ベンザルコニウム、塩化ベンゼトニウム、塩化ジデシルジメチルアンモニウムが例示される。【選択図】図2