An exemplary system includes a scene processing module configured to receive a single frame comprising a first set of pixel data comprising a first combined scene including a fluorescence scene component and a second set of pixel data comprising a second combined scene including a combination of a visible color component scene and the fluorescence scene component. The scene processing module is further configured to extract a display fluorescence scene component from the first combined scene and extract a display visible scene component from the second combined scene. The system further includes a display unit configured to generate, based on the display fluorescence scene component and the display visible scene component, a displayed scene.
