PROBLEM TO BE SOLVED: To provide a method for efficiently making a liver tissue having liver activity.SOLUTION: A method of making a three-dimensional liver tissue includes inoculating a biodegradable scaffold material with a hepatocyte, an endothelial cell, and a mesenchymal stem cell, and coculturing them in the scaffold material under a simulated microgravity gravity environment.SELECTED DRAWING: NoneCOPYRIGHT: (C)2017,JPO&INPIT【課題】肝臓活性を有する肝臓組織を効率よく作製する方法の提供。【解決手段】肝細胞、内皮細胞、及び間葉系幹細胞を、生分解性の足場材料に播種し、その足場材料内で擬微小重力環境下にて共培養することを含む、3次元肝臓組織の作製方法。【選択図】なし