PROBLEM TO BE SOLVED: To provide a method for manufacturing a transparent conductive substrate in which a transparent conductive layer and a metal layer can be favorably patterned without degrading characteristics of the transparent conductive layer containing metal fiber.SOLUTION: The method for manufacturing a transparent conductive substrate includes the steps of: preparing a laminate comprising a transparent substrate and a transparent conductive layer containing metal fiber that comprises at least either silver or copper, formed on the substrate; forming a metal layer containing at least either titanium or aluminum on the transparent conductive layer; forming a resist pattern on the metal layer; etching the metal layer and the transparent conductive layer; removing the resist pattern; forming a protective layer on the metal layer in an outer peripheral region of the transparent substrate; etching only the metal layer by using a solution containing phosphoric acid and ammonium fluoride; and removing the p