The present invention relates to a garlic formulation enriched with sulphur containing amino acids and a process for enriching sulphur containing amino acids in garlic. The sulphur containing amino acids includes S-allylcysteine, S-methylcysteine, and S-allylmercaptocysteine. The percentage of enriched garlic concentrate after processing is in the range of 7.5-9.5%. The garlic formulation is useful in the treatment of Diabetes Mellitus (DM) and prevention of tissue and organ damage that occurs frequently in diabetic patients.