The diagnosability of diseased parts is improved by precisely correcting fluorescence images without being affected by specular reflection and without being affected by thick blood vessels. Provided is a fluorescence observation apparatus (1) that includes a light source unit (2) that radiates, onto an observation target site (S), illumination light that is in the wavelength band absorbed by a blood vessel, first excitation light that includes the wavelength band of the illumination light and generates autofluorescence by exciting an autofluorescent substance in the blood vessel, and second excitation light that is in a different wavelength band from the first excitation light and generates agent fluorescence by exciting fluorescent dye that selectively stains normal tissue and abnormal tissue an image-acquisition unit (15, 16) that obtains respective images by capturing reflected light, autofluorescence, and agent-fluorescence, obtained by radiating the illumination light, and the first and the second excitation light onto the same observation target site (S) and an image processing unit (4) that extracts a blood-vessel image from the obtained reflected-light image, obtains a reference image by applying interpolation to an area in the autofluorescence image corresponding to the extracted blood-vessel image, and corrects the agent-fluorescence image, obtained by the image-acquisition unit, based on the obtained reference image.
