The present invention provides [1] a method for producing a retinal tissue, including the following steps (1) to (3): (1) a first step of subjecting pluripotent stem cells to floating culture in a serum-free medium containing a substance inhibiting the Wnt signal pathway to form an aggregate of pluripotent stem cells, (2) a second step of subjecting the aggregate formed in the first step to floating culture in a serum-free medium containing a basement membrane preparation, and (3) a third step of subjecting the aggregate cultured in the second step to floating culture in a serum-containing medium； [2] a method for producing an optic-cup-like structure, including a step of subjecting an aggregate containing the retinal tissue cultured in the step (3) above to floating culture in a serum-free medium or serum-containing medium each containing a substance acting on the Sonic hedgehog signal pathway and a substance acting on the Wnt signal pathway； [3] a method for producing a retinal pigment epithelium, including a step of subjecting an aggregate containing the retinal tissue cultured in the step (3) above to floating culture in a serum-free medium or serum-containing medium each containing a substance acting on the Wnt signal pathway (wherein said serum-free medium and serum-containing medium are free of a substance acting on the Sonic hedgehog signal pathway)； and [4] a method for producing a retinal layer-specific neural cell, including bringing a retinal progenitor cell contained in a retinal tissue derived from a primate pluripotent stem cell into contact with a substance inhibiting the Notch signal pathway.본 발명은, [1] 하기 (1) 내지 (3) 의 단계를 포함하는 것을 특징으로 하는 망막 조직의 제조 방법: (1) 다능성 줄기 세포를 Wnt 신호전달 경로 저해 물질을 함유하는 무혈청 배지 중에 부유 배양함으로써 다능성 줄기 세포의 응집체를 형성시키는 제 1 단계, (2) 제 1 단계에서 형성된 응집체를 기저막 제제를 포함하는 무혈청 배지 중에 부유 배양하는 제 2 단계, 및 (3) 제 2 단계에서 배양된 응집체를 혈청 배지 중에 부유 배양하는 제 3 단계； [2] 단계 (3) 에서 배양된 응집체를 각각 Sonic hedgehog 신호전달 경로 작용 물질 및 Wnt 신호전달 경로 작용 물질을 함유하는 무혈청 배지 중
